Selected article for: "flow cytometry and room temperature"
Author: Jacob W. Myerson; Priyal N. Patel; Nahal Habibi; Landis R. Walsh; Yi-Wei Lee; David C. Luther; Laura T. Ferguson; Michael H. Zaleski; Marco E. Zamora; Oscar A. Marcos-Contreras; Patrick M. Glassman; Ian Johnston; Elizabeth D. Hood; Tea Shuvaeva; Jason V. Gregory; Raisa Y. Kiseleva; Jia Nong; Kathryn M. Rubey; Colin F. Greineder; Samir Mitragotri; George S. Worthen; Vincent M. Rotello; Joerg Lahann; Vladimir R. Muzykantov; Jacob S. Brenner
Title: Supramolecular Organization Predicts Protein Nanoparticle Delivery to Neutrophils for Acute Lung Inflammation Diagnosis and Treatment
  • Document date: 2020_4_18
    • ID: ezrkg0dc_53
    Snippet: First, fluorescent LDNGs were added to single cell suspensions prepared from human lungs. 5 µg, 10 µg, or 50 µg of LDNGs were incubated with 6x10 5 cells in suspension for 1 hour at room temperature. After three washes to remove unbound LDNGs, cells were stained for CD45 and analyzed with flow cytometry ( Figure 7A -B). The majority of LDNG uptake in the single cell suspensions was attributable to CD45positive cells. LDNGs accumulated in the h.....
    Document: First, fluorescent LDNGs were added to single cell suspensions prepared from human lungs. 5 µg, 10 µg, or 50 µg of LDNGs were incubated with 6x10 5 cells in suspension for 1 hour at room temperature. After three washes to remove unbound LDNGs, cells were stained for CD45 and analyzed with flow cytometry ( Figure 7A -B). The majority of LDNG uptake in the single cell suspensions was attributable to CD45positive cells. LDNGs accumulated in the human leukocytes, extracted from inflamed lungs, in a dose-dependent manner, with 35.1% of leukocytes containing LDNGs at a loading dose of 50 µg. Therefore, our prototype NAP was retained in leukocytes from human lungs.

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