Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_10
Snippet: The loss of the 62kDa PARP2 signal in cells cultured in SF medium prompted us 272 to undertake a more detailed investigation on the relationship between serum treatment and 273 the levels of PARP2 (Fig. 2B ). COS-7 cells were cultured in SF medium or in medium 274 containing increasing amounts of serum. Immunoblot analysis showed a similar staining 275 pattern for full-length PARP1 and its C-terminal fragment, regardless of whether serum 276 was .....
Document: The loss of the 62kDa PARP2 signal in cells cultured in SF medium prompted us 272 to undertake a more detailed investigation on the relationship between serum treatment and 273 the levels of PARP2 (Fig. 2B ). COS-7 cells were cultured in SF medium or in medium 274 containing increasing amounts of serum. Immunoblot analysis showed a similar staining 275 pattern for full-length PARP1 and its C-terminal fragment, regardless of whether serum 276 was present or not (Fig. 2B ). In contrast, increasing the serum concentration from 0 to 10% 277 resulted in the significant increase in the appearance of PARP2 (P<0.05; Fig. 2B ). Together 278 these results showed that PARP2, but not PARP1, changes in cells in response to serum. 279 Furthermore, the evidence with that seen in figures 1C and 1D, would suggest that this 280 increase is at the protein level rather than due to increased gene activity. 281
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