Selected article for: "gene sequence and genome sequence"

Author: Yonghua Wu
Title: Strong evolutionary convergence of receptor-binding protein spike between COVID-19 and SARS-related coronaviruses
  • Document date: 2020_3_4
  • ID: 4ihv80au_14
    Snippet: We used 35 coronavirus strain genomes of the subgenus Sarbecovirus based on two published studies 5,6 , including 2019-nCoV, SARS-CoV, and their phylogenetic relative, bat SARS-like CoV (please see Fig. 1 for details). For all these coronavirus strains, we downloaded their full-length genome sequences from GenBank except for five 2019-nCoV and RaTG13 strains, which were downloaded from GISAID. We aligned these genome sequences using MAFFT (https:.....
    Document: We used 35 coronavirus strain genomes of the subgenus Sarbecovirus based on two published studies 5,6 , including 2019-nCoV, SARS-CoV, and their phylogenetic relative, bat SARS-like CoV (please see Fig. 1 for details). For all these coronavirus strains, we downloaded their full-length genome sequences from GenBank except for five 2019-nCoV and RaTG13 strains, which were downloaded from GISAID. We aligned these genome sequences using MAFFT (https://mafft.cbrc.jp/alignment/server/). The coding sequences of 11 genes (Fig. 1 ) annotated in the genome of 2019-nCoV (NC_045512) were used as a reference sequence to obtain the homologous gene sequences of the 35 coronavirus strains. We aligned these homologous gene sequences using the online software webPRANK (http://www.ebi.ac.uk/goldman-srv/webprank/) 38 , which is considered to create a more reliable alignment to decrease false-positive results in positive selection analyses 39 .

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