Author: Lucia Grenga; Fabrice Gallais; Olivier Pible; Jean-Charles Gaillard; Duarte Gouveia; Hélène Batina; Niza Bazaline; Sylvie Ruat; Karen Culotta; Guylaine Miotello; Stéphanie Debroas; Marie-Anne Roncato; Gérard Steinmetz; Charlotte Foissard; Anne Desplan; Béatrice Alpha-Bazin; Christine Almunia; Fabienne Gas; Laurent Bellanger; Jean Armengaud
Title: Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines Document date: 2020_4_17
ID: kd02dehk_32
Snippet: Overrepresented proteins were described by a larger variety of peptides. Interestingly, an increase of protein levels relied on the increasing abundance of the same set of peptides with only a few new sequences registered at late time points suggesting that absolute quantification of the virus could be obtained by targeted approaches by following early detectable peptides. Eventually tandem mass spectrometry proteotyping [24, 25] could be also pr.....
Document: Overrepresented proteins were described by a larger variety of peptides. Interestingly, an increase of protein levels relied on the increasing abundance of the same set of peptides with only a few new sequences registered at late time points suggesting that absolute quantification of the virus could be obtained by targeted approaches by following early detectable peptides. Eventually tandem mass spectrometry proteotyping [24, 25] could be also proposed to detect SARS-CoV-2 viruses.
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