Selected article for: "infectious virus and virus titer"

Author: Lucia Grenga; Fabrice Gallais; Olivier Pible; Jean-Charles Gaillard; Duarte Gouveia; Hélène Batina; Niza Bazaline; Sylvie Ruat; Karen Culotta; Guylaine Miotello; Stéphanie Debroas; Marie-Anne Roncato; Gérard Steinmetz; Charlotte Foissard; Anne Desplan; Béatrice Alpha-Bazin; Christine Almunia; Fabienne Gas; Laurent Bellanger; Jean Armengaud
Title: Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines
  • Document date: 2020_4_17
  • ID: kd02dehk_8
    Snippet: Vero E6 (ATCC, CLR-1586) cells were cultured at 37°C in 9% CO2 in Dulbecco's modified Eagle's medium (DMEM, Gibco™, ThemoFisher) supplemented with 5% fetal calf serum (FCS) and 0.5% penicillinstreptomycin. Cells were passaged by trypsinization every 2 days. The SARS-CoV-2 strains 2019-nCoV/Italy-INMI1 (Genbank MT066156) was provided by the Lazzaro Spallanzani National Institute of Infectious Diseases (Rome, Italy) via the EVAg network (Europea.....
    Document: Vero E6 (ATCC, CLR-1586) cells were cultured at 37°C in 9% CO2 in Dulbecco's modified Eagle's medium (DMEM, Gibco™, ThemoFisher) supplemented with 5% fetal calf serum (FCS) and 0.5% penicillinstreptomycin. Cells were passaged by trypsinization every 2 days. The SARS-CoV-2 strains 2019-nCoV/Italy-INMI1 (Genbank MT066156) was provided by the Lazzaro Spallanzani National Institute of Infectious Diseases (Rome, Italy) via the EVAg network (European Virus Archive goes global). SARS-CoV-2 stocks used in the experiments had undergone two passages on Vero E6 cells and were stored at -80°C. Virus titer was determined by standard plaque assay (1x10 7 pfu/ml).

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