Author: Maximilian Krause; Adnan M. Niazi; Kornel Labun; Yamila N. Torres Cleuren; Florian S. Müller; Eivind Valen
Title: tailfindr: Alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing Document date: 2019_3_25
ID: cq7g8azh_14
Snippet: Next to handle data from ONT RNA sequencing applications, tailfindr is the first tool to show that poly(A) tails can be measured in ONT DNA sequencing. We further modified tailfindr to handle the most up-to-date basecalling approaches with Flip-flop models. We could show that poly(A) tail measurements from ONT DNA sequencing are more precise compared to measurements of similar RNA molecules (Fig. 2D) . This is mainly explained by a faster and mor.....
Document: Next to handle data from ONT RNA sequencing applications, tailfindr is the first tool to show that poly(A) tails can be measured in ONT DNA sequencing. We further modified tailfindr to handle the most up-to-date basecalling approaches with Flip-flop models. We could show that poly(A) tail measurements from ONT DNA sequencing are more precise compared to measurements of similar RNA molecules (Fig. 2D) . This is mainly explained by a faster and more robust translocation rate with less likelihood for stochastic stalling during sequencing. Interestingly, poly(T) stretches are commonly estimated slightly longer than poly(A) stretches from the same sample (Fig. 2C) . A possible explanation for this would be a slightly faster translocation speed of purine stretches compared to pyrimidine stretches, and could be accounted for in more specific normalisation strategies in the future.
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