Susceptibility of ferrets, cats, dogs, and different domestic animals to SARS-coronavirus-2

Hualan Chen

To access the susceptibility of dogs, pigs, chickens, and ducks to SARS-CoV-2, three-month-old beagles The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.30.015347 doi: bioRxiv preprint ducks) of CTan-H, and two (dogs) or three (pigs, chickens, ducks) uninfected animals were housed in the same room with their infected counterparts to monitor the transmission of CTan-H. Oropharyngeal and rectal swabs from all animals were collected every other day for viral RNA detection. One beagle was viral RNA positive by its rectal swab on day 2 p.i. and was euthanized on day 4 p.i.; its organs, including nasal turbinates, soft palates, tonsils, tracheas, lungs, brains, hearts, kidneys, spleens, livers, pancreas, and small intestines, were collected for viral RNA detection by qPCR. Sera were collected from all remaining animals on day 14 p.i., and antibodies against SARS-CoV-2 were detected by using a Double Antigen Sandwich ELISA kit (ProtTech, Luoyang, China).