Author: Maximilian Krause; Adnan M. Niazi; Kornel Labun; Yamila N. Torres Cleuren; Florian S. Müller; Eivind Valen
Title: tailfindr: Alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing Document date: 2019_3_25
ID: cq7g8azh_22
Snippet: Native RNA Sequencing was performed using the ONT kit SQK-RNA001 following the manufacturer's protocol. In brief, 500 ng of poly(A)-selected RNA were mixed with 100 ng of poly(A) spike-in RNA, or 500 ng poly(A) spike-in RNA were used alone. The RNA was ligated to ONT RT adapter (RTA) and used for Reverse Transcription with SuperScript II (ThermoFisher, #18064022). Next, the proprietary Sequencing Adapter was ligated using T4 DNA ligase (NEB,.....
Document: Native RNA Sequencing was performed using the ONT kit SQK-RNA001 following the manufacturer's protocol. In brief, 500 ng of poly(A)-selected RNA were mixed with 100 ng of poly(A) spike-in RNA, or 500 ng poly(A) spike-in RNA were used alone. The RNA was ligated to ONT RT adapter (RTA) and used for Reverse Transcription with SuperScript II (ThermoFisher, #18064022). Next, the proprietary Sequencing Adapter was ligated using T4 DNA ligase (NEB,
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