Alpha Herpesvirus Egress and Spread from Neurons Uses Constitutive Secretory Mechanisms Independent of Neuronal Firing Activity

Anthony E. Ambrosini; Nikhil Deshmukh; Michael J. Berry; Lynn W. Enquist; Ian B. Hogue

167 20 min. beginning at 10 hpi (Fig 2B-C) . We also performed plaque assays 168 measuring virus titer in the neurite compartment ( Fig 2D) . The first fluorescent . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/729830 doi: bioRxiv preprint 169 capsid protein (mRFP-VP26) expression was detected by 16 hpi in all conditions 170 (Fig 2B) , and we did not observe any differences in the kinetics of spread over 171 time (Fig 2C) . TTX treatment had no effect on virus titer in the neurite 172 compartment at 17 or 24 hpi, and KCl treatment caused an ~1 log reduction in 173 neurite compartment titer at 17 hpi.