Author: Brian G. Pierce; Zhen-Yong Keck; Ruixue Wang; Patrick Lau; Kyle Garagusi; Khadija Elkholy; Eric A. Toth; Richard A. Urbanowicz; Johnathan D. Guest; Pragati Agnihotri; Melissa C. Kerzic; Alexander Marin; Alexander K. Andrianov; Jonathan K. Ball; Roy A. Mariuzza; Thomas R. Fuerst; Steven K.H. Foung
Title: Structure-based design of hepatitis C virus E2 glycoprotein improves serum binding and cross-neutralization Document date: 2020_4_17
ID: b6to1v4u_59
Snippet: Domain-specific serum binding was tested using ELISA with C-terminal biotinylated peptides from H77C AS412 (aa 410-425; sequence NIQLINTNGSWHINST) and AS434 (aa 434-446; sequence NTGWLAGLFYQHK), using 2 μg/ml coating concentration. Recombinant sE2 and E1E2 proteins were captured onto GNA-coated microtiter plates. Endpoint titers were calculated by curve fitting in GraphPad Prism software, with endpoint OD defined as four times the highest absorb.....
Document: Domain-specific serum binding was tested using ELISA with C-terminal biotinylated peptides from H77C AS412 (aa 410-425; sequence NIQLINTNGSWHINST) and AS434 (aa 434-446; sequence NTGWLAGLFYQHK), using 2 μg/ml coating concentration. Recombinant sE2 and E1E2 proteins were captured onto GNA-coated microtiter plates. Endpoint titers were calculated by curve fitting in GraphPad Prism software, with endpoint OD defined as four times the highest absorbance value of Day 0 sera.
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