Author: NISREEN M.A. OKBA; Marcel A Muller; Wentao Li; Chunyan Wang; Corine H. GeurtsvanKessel; Victor M. Corman; Mart M. Lamers; Reina S. Sikkema; Erwin de Bruin; Felicity D. Chandler; Yazdan Yazdanpanah; Quentin Le Hingrat; Diane Descamps; Nadhira Houhou-Fidouh; Chantal B. E. M. Reusken; Berend-Jan Bosch; Christian Drosten; Marion P.G. Koopmans; Bart L. Haagmans
Title: SARS-CoV-2 specific antibody responses in COVID-19 patients Document date: 2020_3_20
ID: 9595vm0k_39
Snippet: We further validated beta-versions of an IgA and an IgG S1 commercial ELISA in two different labs. While the IgA-based ELISA showed higher sensitivity than the IgG-based ELISA, the opposite was true for the specificity where the IgG ELISA was more specific than the IgA ELISA. Yet, we noted some cross reactivity in both ELISAs with serum samples from the same two HCoV-OC43 patients that cross reacted in a MERS-CoV S1 IgG ELISA (6) despite the diff.....
Document: We further validated beta-versions of an IgA and an IgG S1 commercial ELISA in two different labs. While the IgA-based ELISA showed higher sensitivity than the IgG-based ELISA, the opposite was true for the specificity where the IgG ELISA was more specific than the IgA ELISA. Yet, we noted some cross reactivity in both ELISAs with serum samples from the same two HCoV-OC43 patients that cross reacted in a MERS-CoV S1 IgG ELISA (6) despite the different antigen coated. This indicates a response to another protein which could be in the blocking or coating matrix, apart from the specific antigen coated, resulting in this consistent false positive result. All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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