Systemic analysis of tissue cells potentially vulnerable to SARS-CoV-2 infection by the protein-proofed single-cell RNA profiling of ACE2, TMPRSS2 and Furin proteases

Lulin Zhou; Zubiao Niu; Xiaoyi Jiang; Zhengrong Zhang; You Zheng; Zhongyi Wang; Yichao Zhu; Lihua Gao; Xiaoning Wang; Qiang Sun

For scRNA profiling, the raw gene expression matrices for single cells were downloaded from Gene Expression Omnibus (GEO) database(https://www.ncbi.nlm.nih.gov/geo/) and Human Cell Atlas Data Portal (https://data.humancellatlas.org/). We totally acquired the single cell gene expression datasets of various normal tissues and organs, including lung, heart, liver, kidney, intestine, stomach, testis, ovary, adrenal gland and thyroid. Apart from the heart dataset that was obtained by the smart-seq2 method, a majority of the single cell data were generated through the 10x Chromium platform. The GEO accession number of these datasets respectively is GSE122960(lung) [26] , GSE109816(heart) [27] , GSE131685(kidney) [28] , GSE125970(intestine) [29] , GSE134520(stomach) [30] , GSE109037(testis) [31] , GSE134355(thyroid and adrenal gland) [32] , and GSE118127(ovary) [33] . The liver dataset was acquired from the Human Cell Atlas Data Portal [34] .