Selected article for: "dna kit and gene expression library"
Author: Guangchun Han; Ansam Sinjab; Warapen Treekitkarnmongkol; Patrick Brennan; Kieko Hara; Kyle Chang; Elena Bogatenkova; Beatriz Sanchez-Espiridion; Carmen Behrens; Boning Gao; Luc Girard; Jianjun Zhang; Boris Sepesi; Tina Cascone; Lauren Byers; Don L. Gibbons; Jichao Chen; Seyed Javad Moghaddam; Edwin J. Ostrin; Junya Fujimoto; Jerry Shay; John V. Heymach; John D. Minna; Steven Dubinett; Paul A. Scheet; Ignacio I. Wistuba; Edward Hill; Shannon Telesco; Christopher Stevenson; Avrum E. Spira; Linghua Wang; Humam Kadara
Title: Single-cell analysis of human lung epithelia reveals concomitant expression of the SARS-CoV-2 receptor ACE2 with multiple virus receptors and scavengers in alveolar type II cells
  • Document date: 2020_4_17
    • ID: j3vruni3_11
    Snippet: Up to 10,000 cells per sample were partitioned into nanoliter-scale Gel beads-in-emulsion (GEMs) using Chromium Next GEM Single Cell 5' Gel Bead Kit v1.1 (1000169, 10X Genomics) and by loading onto Chromium Next GEM Chips G (1000127, 10X Genomics). GEMs were then recovered to construct single-cell 5' gene expression libraries using the Chromium Next GEM Single Cell 5' Library kit (1000166, 10X Genomics) according to the manufacturer's protocol. B.....
    Document: Up to 10,000 cells per sample were partitioned into nanoliter-scale Gel beads-in-emulsion (GEMs) using Chromium Next GEM Single Cell 5' Gel Bead Kit v1.1 (1000169, 10X Genomics) and by loading onto Chromium Next GEM Chips G (1000127, 10X Genomics). GEMs were then recovered to construct single-cell 5' gene expression libraries using the Chromium Next GEM Single Cell 5' Library kit (1000166, 10X Genomics) according to the manufacturer's protocol. Briefly, recovered barcoded GEMs were broken and pooled, followed by magnetic bead clean-up (Dynabeads MyOne Silane, 37002D, Thermo Fisher Scientific). 10X-barcoded full-length cDNA was then amplified via PCR and analyzed using Bioanalyzer High Sensitivity DNA kit (5067-4626, Agilent). Up to 50 ng of cDNA was carried over to construct gene expression libraries and was enzymatically fragmented and size-selected to optimize the cDNA amplicon size prior to 5' gene expression library construction.

    Search related documents:
    Co phrase search for related documents
    • amplicon size and gene expression: 1
    • amplicon size and High sensitivity: 1
    • Bead kit and dna kit: 1, 2, 3
    • Bead kit and Gel Bead kit: 1, 2, 3
    • Bead kit and High sensitivity: 1
    • Bead kit and High sensitivity dna kit: 1
    • dna kit and Gel Bead kit: 1
    • dna kit and gene expression: 1, 2, 3, 4, 5
    • dna kit and gene expression library: 1
    • dna kit and High sensitivity: 1, 2, 3, 4
    • dna kit and High sensitivity dna kit: 1, 2, 3, 4
    • Gel Bead kit and High sensitivity: 1
    • Gel Bead kit and High sensitivity dna kit: 1
    • gene expression and High sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • gene expression and High sensitivity dna kit: 1
    • gene expression library and High sensitivity: 1
    • gene expression library and High sensitivity dna kit: 1