Author: Brierley, Ian; Digard, Paul; Inglis, Stephen C.
Title: Characterization of an efficient coronavirus ribosomal frameshifting signal: Requirement for an RNA pseudoknot Cord-id: 5i3g2zb7 Document date: 1989_5_19
ID: 5i3g2zb7
Snippet: Abstract The genomic RNA of the coronavirus IBV contains an efficient ribosomal frameshifting signal at the junction of two overlapping open reading frames. We have defined by deletion analysis an 86 nucleotide sequence encompassing the overlap region which is sufficient to allow frameshifting in a heterologous context. The upstream boundary of the signal consists of the sequence UUUAAAC, which is the likely site of ribosomal slippage. We show by creation of complementary nucleotide changes that
Document: Abstract The genomic RNA of the coronavirus IBV contains an efficient ribosomal frameshifting signal at the junction of two overlapping open reading frames. We have defined by deletion analysis an 86 nucleotide sequence encompassing the overlap region which is sufficient to allow frameshifting in a heterologous context. The upstream boundary of the signal consists of the sequence UUUAAAC, which is the likely site of ribosomal slippage. We show by creation of complementary nucleotide changes that the RNA downstream of this “slippery†sequence folds into a tertiary structure termed a pseudoknot, the formation of which is essential for efficient frameshifting.
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