Author: Adriana Larrea-Sarmiento; Anne M. Alvarez; James P. Stack; Mohammad Arif
Title: Synergetic effect of non-complementary 5’ AT-rich sequences on the development of a multiplex TaqMan real-time PCR for specific and robust detection of Clavibacter michiganensis and C. michiganensis subsp. nebraskensis Document date: 2019_3_11
ID: iniz1rjk_24
Snippet: We describe the enhancing effect of 5' AT-rich sequences on reaction efficiency with fast and rapid TaqMan qPCR cycling conditions. The primers and probes were designed using unique and conserved gene regions to detect C. michiganensis species in general as well as specifically, C. m. subsp. nebraskensis. The developed assays are highly sensitive and specific......
Document: We describe the enhancing effect of 5' AT-rich sequences on reaction efficiency with fast and rapid TaqMan qPCR cycling conditions. The primers and probes were designed using unique and conserved gene regions to detect C. michiganensis species in general as well as specifically, C. m. subsp. nebraskensis. The developed assays are highly sensitive and specific.
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