Selected article for: "open reading frame and repeat epitope"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_42
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/478040 doi: bioRxiv preprint nucleotide precision in vitro, until now frameshifting had not been directly observed in a living system. To achieve this, we created a multi-frame repeat epitope tag that can light up single RNA translation sites in different colors depending on which open reading frame is being translated. Together wi.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/478040 doi: bioRxiv preprint nucleotide precision in vitro, until now frameshifting had not been directly observed in a living system. To achieve this, we created a multi-frame repeat epitope tag that can light up single RNA translation sites in different colors depending on which open reading frame is being translated. Together with sensitive single-molecule microscopy and computational modeling, we have demonstrated five novel aspects of HIV-1 frameshifting:

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