Self-assembly of the RZZ complex into filaments drives kinetochore expansion in the absence of microtubule attachment

Cláudia Pereira; Rita M. Reis; José B. Gama; Dhanya K. Cheerambathur; Ana X. Carvalho; Reto Gassmann

Our finding that the expanded domain can be fully detached from the underlying . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/282707 doi: bioRxiv preprint kinetochore, either by acute CDK1 inhibition or by combining GFP::ROD over-expression with Knl1 depletion, suggests that RZZ-dependent expansion results in a distinct multiprotein assembly that is at least transiently stable without the KMN network. Consistent with recent studies [13, 17] , our findings imply that kinetochores contain two distinct pools of Mad1-Mad2: one pool recruited by the KMN network through a direct interaction with Bub1 [7] [8] [9] , and another pool localized to the expanded outer domain, most likely recruited through a direct interaction with RZZ. The presence of BUBR1 and BUB3 on detached crescents may indicate that the expanded domain is able to generate MCC independently of BUB1.