Author: Maximilian Krause; Adnan M. Niazi; Kornel Labun; Yamila N. Torres Cleuren; Florian S. Müller; Eivind Valen
Title: tailfindr: Alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing Document date: 2019_3_25
ID: cq7g8azh_1
Snippet: Oxford Nanopore Technologies (ONT) Sequencing allows for the sequencing of full-length native RNA molecules containing the entire poly(A) tail by ligation of a double-stranded DNA adapter to the 3'-end of each RNA molecule ( Fig. 1A , [30] ). Indeed, long stretches of monotonous low-variance raw signal corresponding to poly(A) tails can be observed at the beginning of most reads (Fig. 1B) . However, since basecalling relies on fluctuations of the.....
Document: Oxford Nanopore Technologies (ONT) Sequencing allows for the sequencing of full-length native RNA molecules containing the entire poly(A) tail by ligation of a double-stranded DNA adapter to the 3'-end of each RNA molecule ( Fig. 1A , [30] ). Indeed, long stretches of monotonous low-variance raw signal corresponding to poly(A) tails can be observed at the beginning of most reads (Fig. 1B) . However, since basecalling relies on fluctuations of the raw signal, these low-variance sections are poorly decoded into the correct nucleobase sequence [31] .
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