Selected article for: "ImageJ software and protein band"
Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress
  • Document date: 2019_6_20
    • ID: dt0b7jnu_61
    Snippet: Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% nonfat milk followed by an overnight incubation with primary antibodies targeting TXNIP (Novus; NBP1-54578), CASP1 (Proteintech; 22915-1-AP), IL1B (Cell Signaling; #12242), or TUBA1B (Epitomics; 1878-1) at 4°C. Following primary antibody incubation, the blots were washed and incubated with HRPconjugated secondary antibodies for one hour (Cell Signaling; #7074S, #7076S). The blots were.....
    Document: Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% nonfat milk followed by an overnight incubation with primary antibodies targeting TXNIP (Novus; NBP1-54578), CASP1 (Proteintech; 22915-1-AP), IL1B (Cell Signaling; #12242), or TUBA1B (Epitomics; 1878-1) at 4°C. Following primary antibody incubation, the blots were washed and incubated with HRPconjugated secondary antibodies for one hour (Cell Signaling; #7074S, #7076S). The blots were then stripped using Restore Western Blot Stripping Buffer (Thermo-Fisher Scientific) and reprobed with alternate antibodies. An antibody against ACTB (Cell Signaling; 8457L) was used as a loading control. Blot imaging was performed on a ChemiDoc MP System (Bio-Rad) after exposing the blot to Clarity Western ECL Blotting Substrate (Bio-Rad). Protein expression was quantitatively analyzed using band density and ImageJ software (NIH, Bethesda MD) (Schneider et al., 2012) .

    Search related documents:
    Co phrase search for related documents
    • antibody incubation and primary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • antibody incubation and primary antibody incubation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • antibody incubation and protein expression: 1
    • antibody incubation and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
    • band density and loading control: 1
    • band density and secondary antibody: 1
    • Bethesda MD NIH imagej software and imagej software: 1
    • Blot imaging and protein expression: 1, 2
    • imagej software and loading control: 1, 2
    • imagej software and nonfat milk: 1
    • imagej software and overnight incubation: 1
    • imagej software and primary antibody: 1, 2, 3
    • imagej software and protein expression: 1, 2
    • imagej software and secondary antibody: 1, 2, 3, 4, 5
    • loading control and overnight incubation: 1, 2
    • loading control and primary antibody: 1, 2
    • loading control and protein expression: 1, 2, 3
    • loading control and secondary antibody: 1, 2, 3, 4, 5, 6, 7
    • nonfat milk and overnight incubation: 1