Selected article for: "secondary antibody and western blotting"
Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress
  • Document date: 2019_6_20
    • ID: dt0b7jnu_61
    Snippet: Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% nonfat milk followed by an overnight incubation with primary antibodies targeting TXNIP (Novus; NBP1-54578), CASP1 (Proteintech; 22915-1-AP), IL1B (Cell Signaling; #12242), or TUBA1B (Epitomics; 1878-1) at 4°C. Following primary antibody incubation, the blots were washed and incubated with HRPconjugated secondary antibodies for one hour (Cell Signaling; #7074S, #7076S). The blots were.....
    Document: Turbo Transfer System (Bio-Rad). Membranes were blocked in 5% nonfat milk followed by an overnight incubation with primary antibodies targeting TXNIP (Novus; NBP1-54578), CASP1 (Proteintech; 22915-1-AP), IL1B (Cell Signaling; #12242), or TUBA1B (Epitomics; 1878-1) at 4°C. Following primary antibody incubation, the blots were washed and incubated with HRPconjugated secondary antibodies for one hour (Cell Signaling; #7074S, #7076S). The blots were then stripped using Restore Western Blot Stripping Buffer (Thermo-Fisher Scientific) and reprobed with alternate antibodies. An antibody against ACTB (Cell Signaling; 8457L) was used as a loading control. Blot imaging was performed on a ChemiDoc MP System (Bio-Rad) after exposing the blot to Clarity Western ECL Blotting Substrate (Bio-Rad). Protein expression was quantitatively analyzed using band density and ImageJ software (NIH, Bethesda MD) (Schneider et al., 2012) .

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