Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress Document date: 2019_6_20
ID: dt0b7jnu_66
Snippet: For luciferase assays, pSG5-PPARA (mouse) and pSG5-RXRA (mouse) were used for transcription factor expression (Shah et al., 2007) . Custom GeneBlocks (IDT DNA) were synthesized containing the predicted PPRE sites for Gm15441. GeneBlocks were digested and purified using a Qiagen PCR Purification Kit (Qiagen, Valencia, CA), and cloned into the pGL4.11.....
Document: For luciferase assays, pSG5-PPARA (mouse) and pSG5-RXRA (mouse) were used for transcription factor expression (Shah et al., 2007) . Custom GeneBlocks (IDT DNA) were synthesized containing the predicted PPRE sites for Gm15441. GeneBlocks were digested and purified using a Qiagen PCR Purification Kit (Qiagen, Valencia, CA), and cloned into the pGL4.11
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