Metabolic Engineering of Fungal Strains for Efficient Production of Cellulolytic Enzymes

Zhao, Xin-Qing; Zhang, Xiao-Yue; Zhang, Fei; Zhang, Ruiqin; Jiang, Bao-Jie; Bai, Feng-Wu

Filamentous fungi are widely used for production of cellulase and other cellulolytic enzymes. Metabolic engineering of filamentous fungal strains has been applied to improve enzyme production, and rapid progress has been made in the recent years. In this chapter, genetic tools and methods to develop superior enzyme producers are summarized, which includes establishment of genetic modification systems, selection and redesign of promoters, and metabolic engineering using either native transcription factors or artificial ones. In addition, enhancement of cellulase production through morphology engineering was also discussed. Emerging tools including CRISPR-Cas9-based genome editing and synthetic biology are highlighted, which are speeding up mechanisms elucidation and strain development, and will further facilitate economic cellulolytic enzyme production.