Author: Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong
Title: Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection Document date: 2019_12_23
ID: ahs2wdus_13
Snippet: The knockout of IFITM3_F01 was generated by a single T base insertion in 174 the first exon using CRISPR/Cas9 in the Kolf2_C1 human iPSC line (a clonal 175 derivative of kolf2 (HipSci)). This was achieved by nucleofection of 10 6 cells 176 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10. 1101 /2019 To differentiate iPSCs to iPSC-derived macrophages (iPSC-Mac), the 193 approach of Hale et al (18) an.....
Document: The knockout of IFITM3_F01 was generated by a single T base insertion in 174 the first exon using CRISPR/Cas9 in the Kolf2_C1 human iPSC line (a clonal 175 derivative of kolf2 (HipSci)). This was achieved by nucleofection of 10 6 cells 176 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10. 1101 /2019 To differentiate iPSCs to iPSC-derived macrophages (iPSC-Mac), the 193 approach of Hale et al (18) and van Wilgenburg et al (19) was modified. 194 Briefly, upon reaching confluency, human iPSCs were collected and 195 transferred into Essential 8 Flex medium supplemented with 50 ng/mL (Bio-Techne), 20 ng/mL SCF (Bio-Techne) and 50 ng/mL VEGF (Peprotech 197 EC Ltd.) in ultra-low attachment plates (Corning) for 4 days to generate 198
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