Author: Ntafis, Vasileios; Xylouri, Eftychia; Mari, Viviana; Papanastassopoulou, Maria; Papaioannou, Nikolaos; Thomas, Angelos; Buonavoglia, Canio; Decaro, Nicola
Title: Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs Cord-id: b7x1mfqc Document date: 2011_10_16
ID: b7x1mfqc
Snippet: In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is charact
Document: In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.
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