Author: Qizhi Sun; Mohamed I. Gatie; Gregory M. Kelly
Title: Serum-dependent and independent regulation of PARP2 Document date: 2018_11_29
ID: ccfrx3md_11
Snippet: Having determined that PARP2 was available as an SDS soluble protein and this 282 was dependent on the presence of serum, the next question was to address how fast the 283 PARP2 signal would decline when cells were deprived of serum. To determine this, COS-284 7 cells were cultured in CM for 24hr until 90% confluence, and then the medium was 285 removed and replaced with SF medium. Cell lysates were collected at 15, 30, 45 and 60 286 minutes and .....
Document: Having determined that PARP2 was available as an SDS soluble protein and this 282 was dependent on the presence of serum, the next question was to address how fast the 283 PARP2 signal would decline when cells were deprived of serum. To determine this, COS-284 7 cells were cultured in CM for 24hr until 90% confluence, and then the medium was 285 removed and replaced with SF medium. Cell lysates were collected at 15, 30, 45 and 60 286 minutes and then processed for immunoblot analysis with the H-250 PARP antibody. 287
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