Selected article for: "1x SDS sample buffer and SDS sample buffer"
Author: Aravinth Kumar Jayabalan; Diane E. Griffin; Anthony K. L. Leung
Title: Alphavirus nsP3 ADP-ribosylhydrolase Activity Disrupts Stress Granule Formation
  • Document date: 2019_6_20
    • ID: n8sjpcbs_50
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/629881 doi: bioRxiv preprint Page 21 fluid was collected in a new tube. The cleared lysates were added to pre-incubated anti-GFP (3E6, Invitrogen)-DYNA magnetic beads (10004D, Invitrogen) complex and incubated for 2 h at RT. The beads were washed once with CLB, twice with high-salt CLB (300 mM NaCl), followed by a final wash with C.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/629881 doi: bioRxiv preprint Page 21 fluid was collected in a new tube. The cleared lysates were added to pre-incubated anti-GFP (3E6, Invitrogen)-DYNA magnetic beads (10004D, Invitrogen) complex and incubated for 2 h at RT. The beads were washed once with CLB, twice with high-salt CLB (300 mM NaCl), followed by a final wash with CLB. The precipitates were then boiled with 1X SDS sample buffer for 10 min at ~85 o C. All experiments were performed at least thrice.

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