Author: Michael M. Lutz; Megan P. Worth; Meleana M. Hinchman; John S. L. Parker; Emily D. Ledgerwood
Title: Mammalian orthoreovirus infection is enhanced in cells pre-treated with sodium arsenite Document date: 2019_2_20
ID: cpfwzudq_12
Snippet: Given the increased percentage of infected cells in dishes pre-treated with sodium 282 arsenite, we tested if pre-treatment of cells with 0.5 mM sodium arsenite for 30 min 283 before reovirus infection enhanced viral yield. We found that pre-treatment with sodium 284 arsenite led to a modest but significant 2-3 fold increase in viral titer (PFU/ml) that was 285 independent of multiplicity of infection, but consistent with the increased numbers of.....
Document: Given the increased percentage of infected cells in dishes pre-treated with sodium 282 arsenite, we tested if pre-treatment of cells with 0.5 mM sodium arsenite for 30 min 283 before reovirus infection enhanced viral yield. We found that pre-treatment with sodium 284 arsenite led to a modest but significant 2-3 fold increase in viral titer (PFU/ml) that was 285 independent of multiplicity of infection, but consistent with the increased numbers of 286 infected cells and the increased protein expression ( Figure 2D ). Together, these data 287 indicate that pre-treatment of cells with sodium arsenite enhances reovirus infectivity by 288 increasing the numbers of virus-permissive cells. 289 290
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