Author: Halaji, Mehrdad; Hashempour, Tayebeh; Moayedi, Javad; Pouladfar, Gholam Reza; Khansarinejad, Behzad; Khashei, Reza; Moattari, Afagh; Musavi, Zahra; Ghassabi, Farzaneh; Pirbonyeh, Neda
Title: Viral etiology of acute respiratory infections in children in Southern Iran. Cord-id: e4ahhhhs Document date: 2019_1_1
ID: e4ahhhhs
Snippet: INTRODUCTION Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Fl
Document: INTRODUCTION Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV. RESULTS Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%. CONCLUSIONS The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
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