Longitudinal virological changes and underlying pathogenesis in hospitalized COVID-19 patients in Guangzhou, China

Li, Zhengtu; Li, Yinhu; Sun, Ruilin; Li, Shaoqiang; Chen, Lingdan; Zhan, Yangqing; Xie, Mingzhou; Yang, Jiasheng; Wang, Yanqun; Zhu, Airu; Gu, Guoping; Yu, Le; Li, Shuaicheng; Liu, Tingting; Chen, Zhaoming; Jian, Wenhua; Jiang, Qian; Su, Xiaofen; Gu, Weili; Chen, Liyan; Cheng, Jing; Zhao, Jincun; Lu, Wenju; Zheng, Jinping; Li, Shiyue; Zhong, Nanshan; Ye, Feng

Prolonged viral RNA shedding and recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in coronavirus disease 2019 (COVID-19) patients have been reported. However, the clinical outcome and pathogenesis remain unclear. In this study, we recruited 43 laboratory-confirmed COVID-19 patients. We found that prolonged viral RNA shedding or recurrence mainly occurred in severe/critical patients (P<0.05). The average viral shedding time in severe/critical patients was more than 50 days, and up to 100 days in some patients, after symptom onset. However, chest computed tomography gradually improved and complete absorption occurred when SARS-CoV-2 RT-PCR was still positive, but specific antibodies appeared. Furthermore, the viral shedding time significantly decreased when the A1,430G or C12,473T mutation occurred (P<0.01 and FDR<0.01) and increased when G227A occurred (P<0.05 and FDR<0.05). High IL1R1, IL1R2, and TNFRSF21 expression in the host positively correlated with viral shedding time (P<0.05 and false discovery rate <0.05). Prolonged viral RNA shedding often occurs but may not increase disease damage. Prolonged viral RNA shedding is associated with viral mutations and host factors. SUPPORTING INFORMATION: The supporting information is available online at 10.1007/s11427-020-1921-5. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.