Selected article for: "expression level and real time"

Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication
  • Document date: 2020_2_10
  • ID: 7n8p9okf_19
    Snippet: Thus, the properties of our target proteases with respect to the S2 pocket were defined at this point as "small" and "covered by a lid" for HCoV-NL63 M pro , "large" and "covered" for SARS-CoV M pro , and "large" and "open" for CVB3 3C pro . Through comparison with crystal structures of other proteases of the same virus genus (HCoV-229E M pro for alphacoronaviruses 28 (PDB entry 1P9S); HKU4 M pro for betacoronaviruses (Ma, Xiao et al., unpublishe.....
    Document: Thus, the properties of our target proteases with respect to the S2 pocket were defined at this point as "small" and "covered by a lid" for HCoV-NL63 M pro , "large" and "covered" for SARS-CoV M pro , and "large" and "open" for CVB3 3C pro . Through comparison with crystal structures of other proteases of the same virus genus (HCoV-229E M pro for alphacoronaviruses 28 (PDB entry 1P9S); HKU4 M pro for betacoronaviruses (Ma, Xiao et al., unpublished; PDB entry 2YNA); and EV-A71 3C pro for enteroviruses 30 (PDB entry 3SGK), we ensured that our conclusions drawn from the template structures were valid for other family members as well.

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