Selected article for: "final volume and room temperature"
Author: Wanchao Yin; Chunyou Mao; Xiaodong Luan; Dan-Dan Shen; Qingya Shen; Haixia Su; Xiaoxi Wang; Fulai Zhou; Wenfeng Zhao; Minqi Gao; Shenghai Chang; Yuan-Chao Xie; Guanghui Tian; He-Wei Jiang; Sheng-Ce Tao; Jingshan Shen; Yi Jiang; Hualiang Jiang; Yechun Xu; Shuyang Zhang; Yan Zhang; H. Eric Xu
Title: Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir
  • Document date: 2020_4_9
    • ID: 7v7pzclb_33
    Snippet: The thermal stability of the RdRp complex was evaluated by using TSA. Briefly, a mixture of 0.5 mg/ml protein and 10× SYPRO Orange (Thermo Fisher Scientific) was incubated for 10 min at room temperature. The reaction was performed in 96-well plates with a final volume of 20 μl. The thermal melting curve were monitored using a LightCycler 480 II Real-Time PCR System (Roche Diagnostics, Rotkreuz, Switzerland) with a ramp rate of 1 °C at the temp.....
    Document: The thermal stability of the RdRp complex was evaluated by using TSA. Briefly, a mixture of 0.5 mg/ml protein and 10× SYPRO Orange (Thermo Fisher Scientific) was incubated for 10 min at room temperature. The reaction was performed in 96-well plates with a final volume of 20 μl. The thermal melting curve were monitored using a LightCycler 480 II Real-Time PCR System (Roche Diagnostics, Rotkreuz, Switzerland) with a ramp rate of 1 °C at the temperature range from 25 °C to 80 °C. The melting peak were calculated by the LightCycler 480 software provided by Roche Diagnostics.

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