Selected article for: "affinity chromatography and chromatography column"
Author: Yan Gao; Liming Yan; Yucen Huang; Fengjiang Liu; Yao Zhao; Lin Cao; Tao Wang; Qianqian Sun; Zhenhua Ming; Lianqi Zhang; Ji Ge; Litao Zheng; Ying Zhang; Haofeng Wang; Yan Zhu; Chen Zhu; Tianyu Hu; Tian Hua; Bing Zhang; Xiuna Yang; Jun Li; Haitao Yang; Zhijie Liu; Wenqing Xu; Luke W. Guddat; Quan Wang; Zhiyong Lou; Zihe Rao
Title: Structure of RNA-dependent RNA polymerase from 2019-nCoV, a major antiviral drug target
  • Document date: 2020_3_17
    • ID: glfxrla9_21
    Snippet: HCl, pH 7.5, 250 mM NaCl and 4 mM MgCl2. Purified nsp12 was concentrated to 4 mg ml/L and stored at 4 °C. The full-length 2019-nCoV nsp7 and nsp8 were co-expressed in E. coil BL21 (DE3) cells as a notagged protein and a 6 × His-SUMO fusion protein, respectively. After purification by Ni-NTA (Novagen) affinity chromatography, the nsp7-nsp8 complex was eluted through on-column tag 20 cleavage by ULP protease. The eluate was further purified by Hi.....
    Document: HCl, pH 7.5, 250 mM NaCl and 4 mM MgCl2. Purified nsp12 was concentrated to 4 mg ml/L and stored at 4 °C. The full-length 2019-nCoV nsp7 and nsp8 were co-expressed in E. coil BL21 (DE3) cells as a notagged protein and a 6 × His-SUMO fusion protein, respectively. After purification by Ni-NTA (Novagen) affinity chromatography, the nsp7-nsp8 complex was eluted through on-column tag 20 cleavage by ULP protease. The eluate was further purified by Hitrap Q ion-exchange column (GE Healthcare, USA) and a Superdex 200 10/300 Increase column (GE Healthcare, USA) in a buffer containing 20 mM Tris-HCl, pH 7.5, 250 mM NaCl and 4 mM MgCl2.

    Search related documents:
    Co phrase search for related documents
    • affinity chromatography and fusion protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • affinity chromatography and increase column: 1
    • affinity chromatography and ion exchange: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
    • affinity chromatography and ion exchange column: 1, 2, 3
    • affinity chromatography and ph HCl: 1, 2, 3, 4, 5, 6
    • affinity chromatography and SUMO fusion protein: 1, 2, 3, 4, 5, 6
    • affinity chromatography and ULP protease: 1
    • affinity chromatography and USA GE Healthcare increase column: 1
    • column tag and fusion protein: 1, 2, 3, 4, 5, 6
    • column tag and ph HCl: 1, 2
    • fusion protein and increase column: 1
    • fusion protein and ion exchange: 1, 2, 3
    • fusion protein and ion exchange column: 1, 2
    • fusion protein and ph HCl: 1, 2, 3, 4, 5, 6
    • fusion protein and SUMO fusion protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • fusion protein and ULP protease: 1
    • fusion protein and USA GE Healthcare increase column: 1
    • increase column and ph HCl: 1
    • increase column and USA GE Healthcare increase column: 1