Author: Ramasubramanian Sundaramoorthy; Amanda L. Hughes; Hassane El-Mkami; David Norman; Tom Owen-Hughes
Title: Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome Document date: 2018_3_30
ID: ct2zhauz_57
Snippet: Xenopus laevis nucleosomes (20nM), reconstituted on Cy3 labelled 0W11 DNA, were bound to titrations of Chd1 enzymes (concentration specified in figure legend) in 50mM Tris pH 7.5, 50mM sodium chloride, and 3mM magnesium chloride supplemented with 100ug/mL BSA. Unbound and bound nucleosomes were separated on a pre-run 6% polyacrylamide gel (49:1 acrylamide: bis-acrylamide) in 0.5X TBE buffer for 1 hour at 150V. The gel shift was scanned on Fujifil.....
Document: Xenopus laevis nucleosomes (20nM), reconstituted on Cy3 labelled 0W11 DNA, were bound to titrations of Chd1 enzymes (concentration specified in figure legend) in 50mM Tris pH 7.5, 50mM sodium chloride, and 3mM magnesium chloride supplemented with 100ug/mL BSA. Unbound and bound nucleosomes were separated on a pre-run 6% polyacrylamide gel (49:1 acrylamide: bis-acrylamide) in 0.5X TBE buffer for 1 hour at 150V. The gel shift was scanned on Fujifilm FLA-5100 imaging system at 532nm.
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