Selected article for: "room temperature and substrate solution"
Author: Brian G. Pierce; Zhen-Yong Keck; Ruixue Wang; Patrick Lau; Kyle Garagusi; Khadija Elkholy; Eric A. Toth; Richard A. Urbanowicz; Johnathan D. Guest; Pragati Agnihotri; Melissa C. Kerzic; Alexander Marin; Alexander K. Andrianov; Jonathan K. Ball; Roy A. Mariuzza; Thomas R. Fuerst; Steven K.H. Foung
Title: Structure-based design of hepatitis C virus E2 glycoprotein improves serum binding and cross-neutralization
  • Document date: 2020_4_17
    • ID: b6to1v4u_67
    Snippet: Serum sample dilutions made in blocking buffer were added to the microwells and incubated for 1 hour at room temperature. Abs were detected with secondary HRP conjugated goat anti-mouse IgG H&L (Abcam, ab97023) and developed with TMB substrate solution (Bio-Rad). The reaction was stopped with 2N sulfuric acid. A Molecular Devices M3 plate reader was used to measure absorbance at 450 nm. Endpoint titers were calculated by curve fitting in GraphPad.....
    Document: Serum sample dilutions made in blocking buffer were added to the microwells and incubated for 1 hour at room temperature. Abs were detected with secondary HRP conjugated goat anti-mouse IgG H&L (Abcam, ab97023) and developed with TMB substrate solution (Bio-Rad). The reaction was stopped with 2N sulfuric acid. A Molecular Devices M3 plate reader was used to measure absorbance at 450 nm. Endpoint titers were calculated by curve fitting in GraphPad Prism software, with endpoint OD defined as four times the highest absorbance value of Day 0 sera.

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