The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19

Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf

Disulfide bonds are important characteristics of protein because they provide stability to the proteins. For this reason, to predict the correct disulfide interactions, the vaccine protein disulfide engineering was carried out with the aid of Disulfide by Design 2 v12.2 (http://cptweb.cpt.wayne.edu/DbD2/) server which predicts the potential sites within a protein structure that have higher possibility of undergoing disulfide bond formation [147] . During disulfide engineering, the intra-chain, inter-chain and Cβ for glycine residue were selected and the χ3 Angle was kept -87° or +97° ± 5 and Cα-Cβ-Sγ Angle was kept 114.6° ±10. The χ3 angle was set at -87° or +97° ± 5 because numerous disulfides were generated by the server, when the default angle (+97° ±30° and -87° ±30°) was used. So, to shorten the amount of disulfides, the χ3 angle was kept low. Furthermore, studies have estimated that Cα-Cβ-Sγ angle reach a peak of near 115°