Author: Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger
Title: Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX Document date: 2018_5_13
ID: 298cbr1x_53
Snippet: The sequence surrounding the cut site in LIMD1 was inserted into a pBSSK (-) backbone using the BamHI and XbaI restriction sites. Mutations were introduced by the Quickchange site directed mutagenesis protocol (Agilent). The 100 nt sequence surrounding the GFP cut site was inserted using the BamHI and XhoI restriction site......
Document: The sequence surrounding the cut site in LIMD1 was inserted into a pBSSK (-) backbone using the BamHI and XbaI restriction sites. Mutations were introduced by the Quickchange site directed mutagenesis protocol (Agilent). The 100 nt sequence surrounding the GFP cut site was inserted using the BamHI and XhoI restriction site.
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