Author: Aaron S. Mendez; Carolin Vogt; Jens Bohne; Britt A. Glaunsinger
Title: Site specific target binding controls RNA cleavage efficiency by the Kaposi’s sarcoma-associated herpesvirus endonuclease SOX Document date: 2018_5_13
ID: 298cbr1x_7
Snippet: This difference was not exclusively due to the fact that the GFP substrate was slightly shorter than LIMD1 54, as SOX also displayed a 2.5-fold reduction of catalytic efficiency on a longer, 100 nt GFP substrate (GFP 100; Fig. 2B ). Electrophoretic mobility shift assays (EMSA) further revealed a 10-fold increase in SOX binding to LIMD1 54 compared to GFP 51 (Fig. 2C) . Given that both substrates contain the requisite unpaired bulge at the predict.....
Document: This difference was not exclusively due to the fact that the GFP substrate was slightly shorter than LIMD1 54, as SOX also displayed a 2.5-fold reduction of catalytic efficiency on a longer, 100 nt GFP substrate (GFP 100; Fig. 2B ). Electrophoretic mobility shift assays (EMSA) further revealed a 10-fold increase in SOX binding to LIMD1 54 compared to GFP 51 (Fig. 2C) . Given that both substrates contain the requisite unpaired bulge at the predicted cleavage site (see Fig. 2A ), these observations suggest that additional sequence or structural features impact SOX targeting efficiency on individual RNAs.
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