Diversity of SARS-CoV-2 genome among various strain identified in Lucknow, Uttar Pradesh

Sahoo, Biswajit; Maurya, Pramod Kumar; Tripathi, Ratnesh Kumar; Agarwal, Jyotsana; Tiwari, Swasti

A new challenge has immerged in the form of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in worldwide. Rapid genome sequencing of SARS-CoV-2 has been a powerful tool to study the pathogenicity of severe acute respiratory syndrome coronavirus 2.During this pandemic situation more genome sequencing of SARS-CoV-2 should be done in order to detect the mutations and genomic modifications across the globe. Here, in this study we have sequenced 23 SARS-CoV-2 positive samples from the state of Uttar Pradesh, India collected during the first pandemic. A range of 2-22 mutations were observed including D614G, L452R, Q613H, Q677H, T1027I in S gene, S194L in N gene, Q57H, L106F, T175I in ORF3a gene as reported previously and also possible novel mutations like P309S in ORF1ab gene, T379I in N gene and L52F, V77I in ORF3a gene were detected.Phylogenetic genome analysis has shown similarity with other SARS-CoV-2 viruses reported in Uttar Pradesh. Mutations in these genes have the potential to affect the severity of the disease. Therefore, identify the mutation is very important to know the pathogenicity of SARS-CoV-2 virus.