Selected article for: "PCR product and purified product"
Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation
  • Document date: 2018_5_17
    • ID: foskvkwn_25
    Snippet: The resulting PCR product was purified using agarose electrophoresis and transformed into K. lactis IFO1267. After five hours of cultivation under nonselective conditions, cells were plated onto solid medium containing G418 (250 µg/ml). Site-specific integration of the PCR cassette into a pGKL2 plasmid as well as the subsequent curing of the unmodified pGKL2 plasmid was evaluated by PCR with primers (in_Kan_rev1 and in_ORF3_forw) and by sequenci.....
    Document: The resulting PCR product was purified using agarose electrophoresis and transformed into K. lactis IFO1267. After five hours of cultivation under nonselective conditions, cells were plated onto solid medium containing G418 (250 µg/ml). Site-specific integration of the PCR cassette into a pGKL2 plasmid as well as the subsequent curing of the unmodified pGKL2 plasmid was evaluated by PCR with primers (in_Kan_rev1 and in_ORF3_forw) and by sequencing of a gel-purified PCR fragment that had been amplified with primers (in_Kan_rev1 and K2_ORF3_for_seq).

    Search related documents:
    Co phrase search for related documents
    • µg ml and gel purify: 1
    • µg ml and PCR cassette: 1
    • µg ml and PCR evaluate: 1, 2
    • µg ml and PCR product: 1, 2, 3
    • µg ml and primer amplify: 1
    • agarose electrophoresis and PCR cassette: 1, 2
    • agarose electrophoresis and PCR evaluate: 1
    • agarose electrophoresis and PCR fragment: 1, 2
    • agarose electrophoresis and PCR product: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • PCR fragment and primer amplify: 1
    • PCR product and primer amplify: 1, 2