Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication Document date: 2020_2_10
ID: 7n8p9okf_60
Snippet: Assessment of inhibitory activity of a-ketoamides using viral replicons and virus-infected cells Cells and viruses. Hepatocellular carcinoma cells (Huh7; ref. 58) and their derivative constitutively expressing T7 RNA polymerase (Huh-T7; ref. 59) were grown in Dulbecco's modified minimal essential medium (DMEM) supplemented with 2 mM glutamine, 100 U·mL -1 penicillin, 100 µg·mL -1 streptomycin sulfate, and fetal calf serum (10% in growth medium.....
Document: Assessment of inhibitory activity of a-ketoamides using viral replicons and virus-infected cells Cells and viruses. Hepatocellular carcinoma cells (Huh7; ref. 58) and their derivative constitutively expressing T7 RNA polymerase (Huh-T7; ref. 59) were grown in Dulbecco's modified minimal essential medium (DMEM) supplemented with 2 mM glutamine, 100 U·mL -1 penicillin, 100 µg·mL -1 streptomycin sulfate, and fetal calf serum (10% in growth medium and 2% in maintenance medium). Huh-T7 cells were additionally supplemented with geneticin (G-418 sulfate, 400 µg·mL -1 ). Huh-T7 cells were used for the enteroviral replicons as well as for infection experiments with CVB3 strain Nancy.
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