Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_51
Snippet: These results provide strong evidence that capped pGKL transcripts contain regular N 7 -methylated caps, just as the endogenous nuclear transcripts represented by actin mRNA in this experiment. In vitro-transcribed actin mRNA that was capped but not N 7 -methylated (G-. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10......
Document: These results provide strong evidence that capped pGKL transcripts contain regular N 7 -methylated caps, just as the endogenous nuclear transcripts represented by actin mRNA in this experiment. In vitro-transcribed actin mRNA that was capped but not N 7 -methylated (G-. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/325316 doi: bioRxiv preprint actin RNA) served as a control of Rai1 activity. Treatment of G-actin RNA by Rai1 and hDcp2 reduced the number of capped transcripts by 43 and 29%, respectively. Conversely, caps of in vitro-transcribed capped and methylated actin RNA (m 7 G-actin) remained completely unaffected by Rai1 treatment, whereas hDcp2 removed 50% of all the caps (Fig. 4B ).
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