Aerosolized Hydrogen Peroxide Decontamination of N95 Respirators, with Fit-Testing and Virologic Confirmation of Suitability for Re-Use During the COVID-19 Pandemic

T. Hans Derr; Melissa A. James; Chad V. Kuny; Prem P. Kandel; Matthew D. Beckman; Kevin L. Hockett; Mark A. Bates; Moriah L Szpara

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.17.20068577 doi: medRxiv preprint . CC-BY-NC-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity. . CC-BY-NC-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity. Figure 1 for titer data). The plate shown in (B) is from the one aHP cycle when dwell time was short and commercial spore-based biological indicators indicated a failure of decontamination. Even on this partial aHP decontamination, only three wells (of which two are shown above) had any viral plaques (indicated by arrowheads; these equate to 25 PFU of HSV-1, and 5 PFU of CVB3). All cycles with successful inactivation of spore-based biological indicators by aHP had complete viral inactivation as well (e.g. top and bottom rows of plate shown in (B)). In contrast, drying on respirators facepieces without aHP (A) only partially inactivates these viruses.