Selected article for: "binding protein and detection limit"
Author: Hsu, You-Ren; Lee, Geng-Yen; Chyi, Jen-Inn; Chang, Chung-ke; Huang, Chih-Cheng; Hsu, Chen-Pin; Huang, Tai-huang; Ren, Fan; Wang, Yu-Lin
Title: Detection of Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein Using AlGaN/GaN High Electron Mobility Transistors
  • Cord-id: mmmwi8de
  • Document date: 2013_3_15
    • ID: mmmwi8de
    Snippet: AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotope-labeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-prote
    Document: AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect the SARS coronavirus (SARS-CoV) nucleocapsid protein interaction without fluorescent labeling. The detection limit in our system was approximately 0.003 nM of protein sample. Our result showed that this technique was more competitive than isotope-labeling EMSA. We demonstrated AlGaN/GaN was highly potential in constructing a semiconductor-based-sensor binding assay to extract the dissociation constants of nucleic acid-protein interaction.

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