Author: Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf
Title: The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19 Document date: 2020_2_11
ID: 7vrm081c_80
Snippet: The GC content of the improved sequence was 51.34% (Figure 11) . The predicted DNA sequence of CV-1 was inserted into the pET-19b vector plasmid between the SgrAI and SphI restriction sites . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint and since the DNA sequence did not have .....
Document: The GC content of the improved sequence was 51.34% (Figure 11) . The predicted DNA sequence of CV-1 was inserted into the pET-19b vector plasmid between the SgrAI and SphI restriction sites . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint and since the DNA sequence did not have restriction sites for SgrAI and SphI restriction enzymes, SgrA1 and SphI restriction sites were conjugated at the N-terminal and C-terminal sites, respectively. The newly constructed vector is illustrated in Figure 12 .
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