tailfindr: Alignment-free poly(A) length measurement for Oxford Nanopore RNA and DNA sequencing

Maximilian Krause; Adnan M. Niazi; Kornel Labun; Yamila N. Torres Cleuren; Florian S. Müller; Eivind Valen

F I G U R E S 1 | Example data output from tailfindr on RNA sequencing data S1A Tabular output containing the unique ONT read ID, tail start and end coordinates, the normaliser (samples_per_nt), the calculated tail length in nt (tail_length) and the filepath for each individual read. S1B Example debugging plots that can be optionally generated by tailfindr for highlighting the poly(A) tail. Displayed is a poly(A) tail of in vitro transcribed eGFP RNA. Top panel shows the total raw data with highlighted poly(A) section (red). Middle panel shows calculated signal derivatives used to define the poly(A) tail boundaries for debugging purposes. Bottom panel displays detected moves as recorded by the basecaller (green).