Author: Jason W. Westerbeck; Carolyn E. Machamer
Title: The Infectious Bronchitis Virus Coronavirus Envelope Protein Alters Golgi pH to Protect Spike Protein and Promote Release of Infectious Virus Document date: 2018_10_11
ID: amg5dice_9
Snippet: We attempted to measure the Golgi luminal pH in cells infected with IBV-EG3, but were unable to achieve a high percent of infected cells in the absence of syncytium formation, since this virus is not efficiently released and spreads best by cell-cell fusion. We found that syncytia were fragile, and this precluded flow cytometric analysis. Instead we turned to transfected cells to determine if the E protein could neutralize the Golgi lumen......
Document: We attempted to measure the Golgi luminal pH in cells infected with IBV-EG3, but were unable to achieve a high percent of infected cells in the absence of syncytium formation, since this virus is not efficiently released and spreads best by cell-cell fusion. We found that syncytia were fragile, and this precluded flow cytometric analysis. Instead we turned to transfected cells to determine if the E protein could neutralize the Golgi lumen.
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