Selected article for: "detection kit and throat swab"
Author: Minfeng Liao; Yang Liu; Jin Yuan; Yanling Wen; Gang Xu; Juanjuan Zhao; Lin Chen; Jinxiu Li; Xin Wang; Fuxiang Wang; Lei Liu; Shuye Zhang; Zheng Zhang
Title: The landscape of lung bronchoalveolar immune cells in COVID-19 revealed by single-cell RNA sequencing
  • Document date: 2020_2_26
    • ID: 8l1vfsbc_40
    Snippet: The throat swab, sputum, nasal swab and BALF were collected from the respiratory tract of the patients at various time-points after hospitalization. Viral RNAs were extracted by Viral RNA was extracted from samples using the QIAamp RNA Viral Kit (Qiagen, Heiden, Germany). Then quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the primers and probes targeting ORF1ab and N genes of SARS-CoV-2 recommended by .....
    Document: The throat swab, sputum, nasal swab and BALF were collected from the respiratory tract of the patients at various time-points after hospitalization. Viral RNAs were extracted by Viral RNA was extracted from samples using the QIAamp RNA Viral Kit (Qiagen, Heiden, Germany). Then quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the primers and probes targeting ORF1ab and N genes of SARS-CoV-2 recommended by China CDC, and a commercial kit specific for SARS-CoV-2 detection (GeneoDX Co., Ltd., Shanghai, China). The specimens were considered tentatively positive if qRT-PCR Ct value lower than 37 is detected at any time-points during the hospitalization. Then the assay is repeated to confirm the positivity if the Ct value was similar or between 37~40. If the repeat Ct was above 40, then the specimen was considered negative.

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