Author: Lyudmila Kovalchuke; Eugene V. Mosharov; Oren A. Levy; Lloyd A. Greene
Title: Stress-induced phospho-ubiquitin formation causes parkin degradation Document date: 2018_12_5
ID: ceepyyxj_86
Snippet: Cell death was assessed as previously described [129] . Briefly, cells were treated with a lysis buffer (150 μL lysis buffer per cm 2 ) that disintegrates the plasma membrane while keeping nuclei intact and counting viable nuclei manually using a hemacytometer. At least 200 nuclei were counted per condition. 10X lysis buffer recipe: 5 g of cetyldimethyl-ethanolammonium bromide, 0.165 g of NaCl, 2.8 ml of glacial acetic acid, 50 ml of 10% Triton .....
Document: Cell death was assessed as previously described [129] . Briefly, cells were treated with a lysis buffer (150 μL lysis buffer per cm 2 ) that disintegrates the plasma membrane while keeping nuclei intact and counting viable nuclei manually using a hemacytometer. At least 200 nuclei were counted per condition. 10X lysis buffer recipe: 5 g of cetyldimethyl-ethanolammonium bromide, 0.165 g of NaCl, 2.8 ml of glacial acetic acid, 50 ml of 10% Triton X-100, 2 ml of 1 M MgCl2, 10 ml of 10X PBS, and 35.2 ml of H2O.
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