Author: Lea Gaucherand; Brittany K. Porter; Summer K. Schmaling; Christopher Harley Rycroft; Yuzo Kevorkian; Craig McCormick; Denys A. Khaperskyy; Marta Maria Gaglia
Title: The influenza A virus endoribonuclease PA-X usurps host mRNA processing machinery to limit host gene expression Document date: 2018_10_14
ID: 8k7w467p_39
Snippet: Beads were resuspended in 50 mM triethylammonium bicarbonate (TEAB) buffer (Sigma). 24 559 mM DTT and 32 mM IAcNH2 were added sequentially. Beads were then incubated for 30 560 minutes at 37°C, washed with 50 mM TEAB and centrifuged for 1 min at 400 x g before 561 resuspending in 50 mM TEAB. On-bead trypsin (PierceTM Trypsin protease, MS-Grade; 562 Thermo Scientific) digest was performed with 1 µg trypsin in 50 mM TEAB buffer, shaken 563 overni.....
Document: Beads were resuspended in 50 mM triethylammonium bicarbonate (TEAB) buffer (Sigma). 24 559 mM DTT and 32 mM IAcNH2 were added sequentially. Beads were then incubated for 30 560 minutes at 37°C, washed with 50 mM TEAB and centrifuged for 1 min at 400 x g before 561 resuspending in 50 mM TEAB. On-bead trypsin (PierceTM Trypsin protease, MS-Grade; 562 Thermo Scientific) digest was performed with 1 µg trypsin in 50 mM TEAB buffer, shaken 563 overnight at 37°C. Samples were acidified with 1 µl trifluoroacetic acid (TFA) and 3 µl Formic 564 acid until a pH lower than 3 was achieved. Trypsinized peptides were collected by puncturing a 565 All rights reserved. No reuse allowed without permission.
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