Author: Wanchao Yin; Chunyou Mao; Xiaodong Luan; Dan-Dan Shen; Qingya Shen; Haixia Su; Xiaoxi Wang; Fulai Zhou; Wenfeng Zhao; Minqi Gao; Shenghai Chang; Yuan-Chao Xie; Guanghui Tian; He-Wei Jiang; Sheng-Ce Tao; Jingshan Shen; Yi Jiang; Hualiang Jiang; Yechun Xu; Shuyang Zhang; Yan Zhang; H. Eric Xu
Title: Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir Document date: 2020_4_9
ID: 7v7pzclb_2
Snippet: For cryo-EM studies, we co-expressed nsp12 with nsp7 and nsp8 to form the core RdRp complex in insect cells ( Figure 1A and Figure S1A -D). Stoichiometry ratio of nsp7 and nsp8 appeared to be less than nsp12, thus additional nsp7 and nsp8 from bacterial expression were supplemented before the final purification step to improve the yield of heterotrimeric complex......
Document: For cryo-EM studies, we co-expressed nsp12 with nsp7 and nsp8 to form the core RdRp complex in insect cells ( Figure 1A and Figure S1A -D). Stoichiometry ratio of nsp7 and nsp8 appeared to be less than nsp12, thus additional nsp7 and nsp8 from bacterial expression were supplemented before the final purification step to improve the yield of heterotrimeric complex.
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